Figure 1From: Immunoglobulin G (IgG) attenuates neuroinflammation and improves neurobehavioral recovery after cervical spinal cord injury IgG crosses the blood spinal cord barrier and associates with astrocytes. (A) Representative fluorescence images showing the presence of IgG in the spinal cords of an injured rat (left) and a non-injured rat (right). (B) Vessels were stained with RECA-1 (green) and IgG was labeled with a human specific IgG secondary antibody (red). Confocal images demonstrate IgG was able to cross the blood-spinal cord barrier in injured animals (left panel) but not in non-injured animals (right panel). (C) Representative confocal images of microglia/macrophages (Iba-1; green) and IgG (red). IgG was observed in the parenchyma surrounding cells marked by Iba-1 and DAPI (blue). Although IgG was in the vicinity of Iba-1 positive cells, co-localization between IgG and Iba-1 was not observed. (D) Representative confocal images of astrocytes (GFAP; green) and IgG (red). IgG was observed in the parenchyma surrounding and in the cell soma of GFAP positive astrocytes. The co-localization of IgG and astrocytes suggests potential interaction between IgG and astrocytes. Note that an IgG-positive signal was not observed in the spinal cord of rats injected with saline. Confocal images (B-D) were taken from the boxed area, and scale bars represent 50 μm in length.Back to article page