IgG reduces IL-1β, IL-6, and MCP-1 expression. Expression of cytokines (TNF-α, IL-1β and IL-6) and chemokines (MCP-1 and CINC-1) at 4 h after SCI was determined with ELISA in the spinal cord homogenates of sham, saline, and IgG-treated animals. SCI resulted in a significant increase of all inflammatory mediators relative to non-injured sham animals. (A) There was no difference in TNF-α protein expression between saline and IgG-treated animals (P = 0.084). (B) IgG treatment was associated with a significant reduction in IL-1β following SCI in rats (P = 0.007). (C) IL-6 protein expression was significantly reduced in IgG-treated animals relative to saline controls (P = 0.003). (D) IgG treatment was associated with a significant decrease in MCP-1 protein expression relative to saline controls (P = 0.003). (E) IgG treatment had no effect on the level of CINC-1 after SCI relative to saline control injected animals (P = 0.38). Error bars represent SEM.