Table 1 List of primary and secondary antibodies used for western blot analysis
Antibody | Host | Company | Dilution | Duration |
|---|---|---|---|---|
Anti-rat β-actin | Mouse | Chemicon (MAB1501R) | 1:500 | Overnight 4°C |
Anti-rat matrix metalloproteinase (MMP)-9 | Rabbit | Chemicon (AB19016) | 1:1,000 | Overnight 4°C |
Anti-rabbit IgG horseradish peroxidase (HRP) | Goat | Jackson Lab (11-035-144) | 1:5,000 | 1 h RT |
Anti-mouse IgG horseradish peroxidase (HRP) | Goat | Sigma (A-3682) | 1:2,000 | 1 h RT |