Table 2 List of primary and secondary antibodies used for immunohistochemistry

Anti-rat ionized calcium binding adapter molecule (Iba)-1

Rabbit

Wako (019–19741)

1:300

Overnight 4°C

Anti-rat endothelial cell antibody (RECA-1)

Mouse

Serotec (MCA970R)

1:100

Overnight 4°C

Anti-human IgG-TRITC

Goat

Chemicon (AP504R)

1:300

1 h RT

Anti-rabbit IgG AlexaFluor 488

Goat

Invitrogen (A11034)

1:300

1 h RT

Anti-mouse IgG AlexaFluor 488

Goat

Invitrogen (A11001)

1:300

1 h RT