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Table 2 List of primary and secondary antibodies used for immunohistochemistry

From: Immunoglobulin G (IgG) attenuates neuroinflammation and improves neurobehavioral recovery after cervical spinal cord injury

Antibody Host Company Dilution Duration
Anti-rat ionized calcium binding adapter molecule (Iba)-1 Rabbit Wako (019–19741) 1:300 Overnight 4°C
Anti-rat endothelial cell antibody (RECA-1) Mouse Serotec (MCA970R) 1:100 Overnight 4°C
Anti-human IgG-TRITC Goat Chemicon (AP504R) 1:300 1 h RT
Anti-rabbit IgG AlexaFluor 488 Goat Invitrogen (A11034) 1:300 1 h RT
Anti-mouse IgG AlexaFluor 488 Goat Invitrogen (A11001) 1:300 1 h RT
  1. Antibody concentrations and incubation conditions are listed.