Minocycline reduces microglial activation after hypoglycemic brain injury. Images show that microglial activation is reduced in the hippocampal CA1 area (CA1) (A) and in the perirhinal cortex area (Ctx) (C) by minocycline treatment after hypoglycemia. Rats received intraperitoneal injections of saline or minocycline (MINO) at 6 hours after hypoglycemia. Injections were continued once per day for 6 days. Microglial activation was evaluated 7 days after hypoglycemia or sham operation. Brain sections from sham-operated (Sham) or hypoglycemic-injured (Hypoglycemia) rats were immunostained with anti-CD11b. Note that activated microglial cells, which showed amoeboid morphology with enlarged soma and thickened processes, were reduced by the delayed treatment with minocycline. Scale bar = 40 μm. Quantification of microglial activation in the hippocampal CA1 area (CA1) (B) and cerebral cortex (Ctx) (D) of rats receiving minocycline (MINO) or vehicle (Veh) after hypoglycemic brain injury. Microglial activation is scored as described in the Methods and Materials. Data are mean ± standard error of the mean (SEM); *P < 0.05 compared with sham-operated animals, #
P < 0.05 compared with injured animals treated with saline (Veh) (n = 6).