Minocycline reduces myeloperoxidase (MPO) immunoreactivity induced by hypoglycemia in the hippocampus. Representative images of reduction in MPO immunoreactive cells in the hippocampal CA1 area (CA1) (A) and in the perirhinal cortex (Ctx) (C) by minocycline treatment after hypoglycemia. Note that hypoglycemia-induced MPO-positive infiltrating cells were reduced by the delayed minocycline treatment. Scale bar = 40 μm. Quantification of MPO-positive cells in the hippocampus (CA1) (B) and in the cortex (Ctx) (D ) of rats treated with minocycline (MINO) or saline (Vehicle) after hypoglycemia. The number of MPO (+) cells was counted under microscope as described in Methods and Materials. Data are mean ± standard error of the mean (SEM); *P < 0.05 compared with sham-operated animals, #
P < 0.05 compared with hypoglycemia animals treated with saline (Vehicle) (n = 7).