Delayed treatment with minocycline has long-term protective effects after hypoglycemia. (A) Representative images of surviving neurons in the post-hypoglycemic hippocampal CA1 area following minocycline treatment. Note that hypoglycemia-induced hippocampal CA1 neuronal death was protected by the delayed minocycline treatment 8 weeks after hypoglycemia. Scale bar = 80 μm. (B) Quantification of surviving neurons in the hippocampal CA1 area of rats treated with minocycline (MINO) or saline (Veh) 8 weeks after hypoglycemia. The number of neurons was counted using the stereology method as described in Methods and Materials. Data are mean ± standard error of the mean (SEM); *P < 0.05 compared with sham-operated animals treated with saline (Veh), #
P < 0.05 compared with hypoglycemia animals treated with saline (n = 6).