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Figure 1 | Journal of Neuroinflammation

Figure 1

From: Interferon regulatory factor 8/interferon consensus sequence binding protein is a critical transcription factor for the physiological phenotype of microglia

Figure 1

IRF8-deficient microglia colonized the CNS normally, but could not be identified by AIF1/IBA1 immunoreactivity. The spinal cords obtained from adult Irf8 +/+ and Irf8 -/- mice were immunolabeled for various microglial markers (A), and positive cells in the dorsal column at the Th1 spinal level were quantified (B). Microglia were reliably identified as CD11b and PU.1 double positive cells in both Irf8 +/+ and Irf8 -/- CNS tissues (pink arrows in far left panels in A). Note that few Irf8 -/- microglia demonstrated detectable immunoreactivity for AIF1/IBA1. Scale bar: 100 μm. **P < 0.01. (C) 20 μm-thick z-stack confocal images of the microglia immunolabeled for CD11b (green) and PU.1 (red) in the dorsal columns at the Th1 spinal level. CD11b-positive microglial processes were much sparser in the dorsal column of Irf8 -/- mice than those in Irf8 +/+ mice, and processes of Irf8 -/- microglia were thicker and less elaborated than those of Irf8 +/+ microglia. PU.1-positive microglial nuclei are indicated by arrows, demonstrating that similar numbers of microglia are distributed in the Irf8 +/+ and Irf8 -/- spinal cords in contrast to the different density of the microglial processes. Scale bar: 100 μm.

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