F4/80, CD11c, and Ly-6G expression in
microglia in the mixed glial cultures maintained in the presence of M-CSF or GM-CSF. Mixed glial cells were cultured in medium alone (control) or medium supplemented with M-CSF (20 ng/ml) or GM-CSF (20 ng/ml) for 4 days, and labeled with anti-CD45 and anti-CD11b antibodies in combination with each of anti-F4/80, anti-CD11c or anti-Ly-6G antibodies. The bivariate plots show expression of CD11b, F4/80, CD11c and Ly-6G on gated CD45-positive cells. The quadrants were set using corresponding isotype controls.