Figure 6

IRF8-deficient microglia demonstrated a reduced phagocytic capacity in vitro. Microglia from Irf8 ^+/+ (A) and Irf8 ^-/- (B) mice were incubated with Alexa Fluor™ 488-conjugated zymosan particles for 24 hours. Scale bar: 50 μm. (C-D) Percentages of Alexa Fluor™ 488-positive cells in total cells (C) and mean fluorescent intensity (MFI) of Alexa Fluor™ 488-positive cells (D) were measured in Irf8 ^+/+, Irf8 ^+/-, and Irf8 ^-/- microglial cultures at 0, 1, 6, and 24 hour after adding the zymosan particles. The MFI of a non-phagocytosed Alexa Fluor™ 488-conjugated zymosan particle is also shown (Zymosan- Alexa488) in D. **P < 0.001 in comparison with the two groups indicated.