AIF1/IBA1 expression in
microglia was reduced but still inducible by IFNγ. (A) Quantitative and kinetic analysis of AIF1/IBA1 mRNA in Irf8
+/+ and Irf8
-/- microglia after addition of medium alone (Control, open circle), IFNγ (IFNG, 100 ng/ml, closed circle), lipopolysaccharide (LPS, 100 ng/ml, open square) or both (closed square). Reverse transcribed cDNA levels at the indicated time points were quantified by qPCR and are plotted as ratios to copy numbers of β-actin cDNA on a logarithmic scale. Data were from at least three independent experiments. At time 0, data from controls are only shown. (B)
-/- microglia expressed far less AIF1/IBA1 protein than Irf8
+/+ microglia. Purified microglia were maintained in the medium containing IFNγ (IFNG, 100 ng/ml), lipopolysaccharide (LPS, 100 ng/ml) or both for 24 h. Subsequent immunoblots for β-actin (ACTB) are shown for equal protein loading.