MAPC treatment increases CD4
cells after TBI in
spleen and blood. (A) Measurement of CD4+/CD25+/FOXP3+ cells was completed in the splenocytes at 24, 48, and 72 hours after CCI injury and CCI injury plus MAPC (n = 4/group). There was a significant increase in T regulatory cells as a percentage of CD4+ T helper cells within the spleens of MAPC treated mice at 24 hours (P ≤0.01), with no difference seen at 48 or 72 hours. (B) In peripheral blood, percent T regulatory cells from MAPC treated mice was significantly higher at 48 hours after CCI injury (P ≤0.01). Of note, there is an additional control of sham (uninjured: white bar) for baseline profile of splenocytes. * represents P <0.05 and ** represents P <0.01. CCI, controlled cortical impact; MAPC, multipotent adult progenitor cell; TBI, traumatic brain injury.