Figure 8

Supernatant derived from MAPC:splencocyte co-cultures promotes anti-inflammatory phenotype of microglia/macrophages. (A) Graph of percentage of M1/M2 activated with LPS and incubated with MAPC:splenocyte co-culture supernatant versus splenocyte culture supernatant (without MAPC). A significant decrease in the double positive microglial cells (CD86⁺ and CD206⁺) is seen in the MAPC:splenocyte co-culture group (P ≤0.05). (B) Fewer CD86⁺ microglia were observed in the MAPC:splenocyte co-culture group, but this change was not significant. (C) Significantly more CD206⁺ cells were observed in the MAPC:splenocyte co-culture group as opposed to the splenocyte alone group (P ≤0.05). (D) Photomicrograph of isolated microglia/macrophages activated LPS and splenocyte alone (without MAPC) supernatant. Note the number of dual stained cells. (E) Photomicrograph of isolated microglia/macrophages activated LPS and incubated with MAPC:splenocyte co-culture supernatant treatment. Note the number of M2 cells (green). M2 cells are labeled green and M1 cells are labeled red. * represents P <0.05. LPS, lipopolysaccharide; MAPC, multipotent adult progenitor cell.