Nestin-cre drives recombination in neurons. (a) Gross images of tdTomato x Nes cre mouse brains showing diffuse expression of the tdTomato protein throughout the central nervous system. Brains from Nes-cre/tdTomato reporter mice were immunostained with antibodies against NeuN labeling neuronal nuclei. Images seen in (b) show high magnification of brains from Nes-cre/tdTomato reporter mice immunostained with antibodies against NeuN to examine co-expression. High power photomicrographs showing cultured cortical neurons from E14.5 Nes-cre/tdTomato mice immunostained with antibodies against neuron-specific proteins NeuN and β3 tubulin (c) or Tau and Map2 (d). Cultured cortical neurons from E14.5 Nes-cre−/−/tdTomato negative control mice stained for Tau and Map2 using the same microscope settings as in (c). Tissue sections from Nes-cre/GNZ mouse hypothalami demonstrating dense nuclear GFP expression (f). Representative images in (f) of the ARC at −1.20 to −1.70 mm relative to bregma. Scale bars, (b) 10 μm, (c, d, e) 50 μm, (f) 100 μm-low power; 50 μm-high power.