MyD88 expression in the CNS is not required for LPS-induced sickness behavior. WT and MyD88ΔCNS mice received i.p. injections of LPS (250 μg/kg) 1 h before the onset of the dark cycle (n = 4 to 9/group). (a) Cumulative food intake. (b) Cumulative food intake at 2 h post injection. (c) Change in body weight after LPS injection. (d) Hourly locomotor activity. (e) Cumulative locomotor activity during the entire night cycle. All data are represented as the mean ± s.e.m. In some cases error bars are too small to see. Lox, MyD88Lox/lox; ΔCNS, MyD88ΔCNS; ZT, Zeitgeber Time. *** , P <0.001 as measured by two-way ANOVA with Bonferroni post-test.