Figure 3

TLR-mediated signaling pathway related gene expression. Real time RT-PCR analysis from the hippocampus revealed that the gene expressions of TLR2 and TLR4 were increased in LPS-vehicle rats (***p < 0.001 and *p < 0.05 vs. aCSF-vehicle rats), while treatment with DT restored expression levels back to control (###p < 0.001 and #p < 0.05 vs. LPS-vehicle). Whereas LPS had no effect on Hmgb1 expression, treatment of LPS infused animals with DT decreased the expression of Hmgb1 (#p < 0.05 vs. LPS-vehicle). Treatment with DT reduced the expression of IRAK1 in both aCSF-DT and LPS-DT rats ($p > 0.05 vs. aCSF-vehicle and #p < 0.05 vs. LPS-vehicle). No significant differences in hippocampal transcript levels of MyD88 or NFκB p65 were observed between the treatment groups.