Figure 1From: Neuronal toll-like receptor 4 signaling induces brain endothelial activation and neutrophil transmigration in vitro Expression of TLR4 mRNA in neuronal and glial cell cultures. Neuronal, mixed glial, microglial and astrocytic primary cultures were analyzed by RT-PCR for expression of TLR4 and GAPDH mRNAs. cDNA amplification was visualized by agarose gel electrophoresis. Images shown are representative of three experiments carried out on separate cultures. GAPDH, glyceraldehyde 3-phosphate dehydrogenase; TLR4, Toll-like receptor 4.Back to article page