A) Mixed glial cultures |
|---|
| |
TNFα
|
RANTES
|
KC
|
IL-6
|
IL-1α
|
IL-1β
|
G-CSF
|
|---|
Untreated | 11 ± 9 | 122 ± 44 | 29 ± 10 | 14 ± 4 | 7 ± 3 | 6 ± 5 | 10 ± 12 |
LPS-treated | 5,673 ± 468***
| 7,805 ± 679***
| 3,974 ± 547***
| 28,945 ± 837***
| 36.7 ± 9.4***
| 98.5 ± 50.7***
| 4,283 ± 832***
|
B) Neuronal cultures
|
| |
TNFα
|
RANTES
|
KC
|
IL-6
|
IL-1α
|
IL-1β
|
G-CSF
|
Untreated | 19 ± 16 | 20 ± 4 | 2 ± 4 | 7 ± 2 | 13 ± 3 | 13 ± 4 | 21 ± 6 |
LPS-treated | 85 ± 12**
| 414 ± 42***
| 549 ± 89***
| 70 ± 12***
| 13 ± 4 | 14 ± 3 | 38 ± 3**
|
- Mixed glial (A) and neuronal (B) primary cultures were treated with LPS (100 ng/ml) for 24 hours, and culture supernatants were assayed for various cytokines and chemokines by CBA analysis. Data are presented as mean ± SD of three independent experiments carried out on separate cultures. **P <0.01, ***P <0.001, LPS-treated versus untreated cultures, using one-way ANOVA and Tukey’s multiple comparison post-hoc test. ANOVA, analysis of variance; CBA, cytometric bead array; G-CSF, colony stimulating factor; KC, CXCL1; LPS, lipopolysaccharide; RANTES, regulated upon activation normal T-cell expressed and presumably secreted; TNFα, tumor necrosis factor-α.
