I-lymphocytes in mice treated with LPS. A 2 × 2 design was used, in which cervical-node lymphocytes from LPS-treated or phosphate buffer-treated (PB) mice were harvested, radioactively labeled, and injected i.v. into mice that were treated with LPS or into untreated mice. Upper left hand panel found no difference in levels of 131I-lymphocytes in the circulation. Upper right panel shows control mice took up more 131I-lymphocytes than did LPS-treated mice, with Mouse accounting for 42% of the statistical variance; these results show that it was the axillary node rather than the lymphocyte that dominated in determining the degree of uptake. Lower left panel shows similar results for spleen, with Mouse accounting for 72% of the variance. Lower right panel shows that LPS cells were taken up to a higher degree by brain. Statistical analysis showed that Cells accounted for 51% of variance, whereas Mouse accounted for 18% and interaction accounted for 12%.