Figure 2

Analysis of nociceptive sensitivity in SJL mice immunized with PLP _139-151 peptide, C57 mice immunized with MOG _35-55 peptide and corresponding control mice. (A, B) time-course of withdrawal latency to radiant heat in (A) SJL-EAE mice (left column, red symbols) and (B) C57-EAE mice (right column, black symbols). (C-H) Comparison of response frequency to von Frey hair filament stimulation. Response frequency toward the application of 0.07 g von Frey hair filament in (C) SJL-EAE and (D) C57-EAE mice, 0.4 g von Frey hair filaments in (E) SJL-EAE and (F) C57-EAE mice, and 1.0 g von Frey hair filament in (G) SJL-EAE and (H) C57-EAE mice. SJL-EAE mice developed major mechanical allodynia in the chronic phase whereas C57-EAE mice showed minor allodynia in the onset and peak phase. n = 6 mice/ group, *P <0.05 as compared to all control groups, ^†as compared to basal values within a group, ANOVA, post hoc Bonferroni’s test. All data points represent mean ± SEM. EAE, experimental autoimmune encephalomyelitis.