Expression of NeuN and Fox3 in lumbar spinal cord in SJL-PLP
peptide immunized mice and control mice over the time course of the EAE. (A) Typical examples of NeuN expression in spinal cord sections of control immunized mice and SJL-EAE mice at 2 to 3 days following immunization (pre-timepoint), in the onset phase, in the peak phase and in the chronic phase of the disease (day 35 to 40 after immunization) showing a strong decrease in NeuN expression. Scale bar represents 1 mm. (B) Examples of dorsal horn spinal cord sections of SJL-EAE mice showing the expression of NeuN (green), Fox3 (red) and the corresponding overlay pictures, indicating a decrease of NeuN antigenicity rather than cellular loss. Scale bar represents 200 μm. EAE, experimental autoimmune encephalomyelitis.