Analysis and quantification of astrocyte and microglia activation in SJL-PLP
peptide immunized mice and C57-MOG
peptide immunized mice. High magnification of lumbar spinal dorsal horn in SJL-EAE and C57-EAE mice at basal level (before immunization), in the onset phase, in the peak phase and in the chronic phase (day 35 to 40 after immunization) of the disease stained with anti-Iba1 (A) or anti-GFAP (B) antibody. SJL-EAE mice showed a stronger activation of Iba1 and GFAP positive cells than C57-EAE mice. Scale bars represent 25 μm in panel A and B. (C,D) Overview of the spinal dorsal horn of SJL-EAE and C57-EAE mice in the chronic phase of the disease showing higher immunoreactivity for Iba1 (C) and GFAP (D). Boxed areas indicate where pictures in panel A and B were taken. Scale bars represent 100 μm in panel C and D. (E,F) Quantification of relative levels of the expression of Iba1 (E) and GFAP (F) proteins in the spinal dorsal horn of SJL-EAE and C57-EAE mice in the chronic phase of the disease. There was a significant higher expression of Iba1 positive cells (E) and GFAP positive cells (F) in SJL-EAE mice as compared to C57-EAE mice. ; EAE, experimental autoimmune encephalomyelitis.