Detectable amounts of intravenous immunoglobulin in the brain and splenocytes of treated mice. Human IgG concentrations were evaluated on splenocytes and in the striatum of intravenous immunoglobulin (IVIg) and control mice. (A) Representative flow cytometric analyses and (B) mean fluorescence intensity (MFI) quantification of extracellular human IgG on CD45+ splenocytes. Note that the MFI of the control groups is equivalent to isotypic control and autofluorescence (data not shown). (C) Concentrations of IVIg were measured in striatum homogenates from intracardially perfused mice using a specific ELISA. (B),(C) Data presented as mean ± standard error of the mean of 12 to 15 animals, ***P <0.001 versus control groups, one-way analysis of variance followed by Tukey’s multiple comparison test.