Figure 2

Expression of PDI and SOD1 in cultured astrocytes following OGD/reperfusion. A. PDI expression in cultured astrocytes showed an increasing trend in the process of reperfusion and peaked at OGD 8 h/reperfusion 24 h. B. SOD1 expression in cultured astrocytes increased gradually during the process of OGD/reperfusion and reached a peak at OGD 8 h/reperfusion 24 h. C. The anti-SOD1 antibody co-precipitated PDI, and the anti-PDI antibody co-precipitated SOD1 in cultured astrocytes. Three separate immunoprecipitations were performed, and data were analyzed in triplicate. Data were presented as mean ± SEM; *, P < 0.05; **, P < 0.01; ***, P < 0.001 by one-way ANOVA (analysis of variance) with Tukey’s post-hoc test.