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Figure 7 | Journal of Neuroinflammation

Figure 7

From: Electroacupuncture pretreatment attenuates cerebral ischemic injury through α7 nicotinic acetylcholine receptor-mediated inhibition of high-mobility group box 1 release in rats

Figure 7

Involvement of α7nAChR in OGD-induced HMGB1 release in primary cultured neurons. (A) Effect of PHA-543,613 and α-BGT on cell viability in normal cultured primary neurons. (B-D) Effects of PHA-543,613 and α-BGT in neurons exposed to OGD on cell viability (B), LDH release (C) and HMGB1 levels (D). The PHA-543,613 (0-12.5 μM) and α-BGT (10 nM) were incubated with primary cultured neurons for 24 h. PHA-543,613 was added immediately before the onset of OGD. The α-BGT was pre-incubated with neurons for 30 min before the onset of OGD. The control cells (0 μM) received vehicle (saline). The cell viability (MTT assay), LDH release and HMGB1 level were tested at 24 h after the exposure of cell cultures to OGD. *P < 0.05 compared with the 0 μM group exposed to OGD.

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