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Table 1 Numbers of animals per group for various experiments at different time points

From: Electroacupuncture pretreatment attenuates cerebral ischemic injury through α7 nicotinic acetylcholine receptor-mediated inhibition of high-mobility group box 1 release in rats

Protocol

Experiment

Time point

Group

Number

Total

Effect of EA pretreatment on α7nAChR expression

Western blot analysis Immunofluorescent staining

24 h, 48 h, 72 h 72 h

Sham, Control, EA

n = 5

n = 3

44

Neuroprotective effect of PHA-543,613 pretreatment

Neurological evaluation and TTC staining

72 h

MCAO, PHA-543,613+MCAO, Vehicle+MCAO

n = 10

30

Effect of α-BGT on EA pretreatment induced neuroprotection and HMGB1 release

Neurological evaluation and TTC staining

72 h

Sham, MCAO, EA+MCAO, α-BGT+EA, Vehicle+EA, α-BGT+MCAO

n = 10

120

 

TUNEL staining

24 h

 

n = 5

 
 

ELISA for HMGB1 level

72 h

 

n = 5

 
  1. EA, electroacupuncture; HMGB1, high mobility group box 1; MCAO, middle cerebral artery occlusion; TTC, 2,3,5-triphenyltetrazolium chloride; TUNEL, terminal deoxynucleotidyl transferase-mediated dUDP-biotin nick end-labeling; α7nAChR, α7 nicotinic acetylcholine receptor; α-BGT, α-bungarotoxin.