Figure 12

Major histocompatibility complex class I (MHC I) and Iba1 double staining in C57BL6/J wild-type (WT) and Toll-like receptor (TLR)2 knockout (KO) mice (TLR2 ^−/− ) mice 1 week after axotomy. (B,E) The contralateral side showed slight IbaI labeling and (A,D) no MHC I in both groups. There was greater upregulation of MHC I (J) in KO mice after lesion compared with (G) WT. (I,L) Colocalization of Iba1 and MHC I labeling, indicating that microglia express MHC I. (M) Graph representing the quantification of the integrated density of pixels for MHC I immunolabeling adjacent to motor neurons. (N) β2-Microglobulin mRNA level in the lumbar spinal cord determined by reverse transcriptase PCR; both graphs show greater MHC I upregulation in TLR2^−/− mice after unilateral sciatic nerve axotomy. * P<0.05. Scale bar: 50 μm.