Figure 2

Representative images of synaptophysin immunostaining in C3H/HePas WT and C3H/HeJ Toll-like receptor (TLR)4 mutant mice at 1 week after unilateral axotomy. Note the stronger synaptic loss in (D) mutant mice compared with (B) WT. (A and C) Unlesioned side of WT and TLR4 mutant mice, respectively. The dashed circle indicates the motor nucleus containing the alpha motor neurons. (E) Graph representing the ipsilateral:contralateral (IL:CL) ratio of the integrated density of pixels. *** P<0.001. (F) Western blot analysis of synaptophysin expression in WT and TLR4 mutant mice. Note the similar expression of synaptophysin between strains. β-Actin was used as sample loading control. Scale bar: 50 μm.