Representative images of glial fibrillary acidic protein (GFAP) immunostaining in C57BL/6J wild-type (WT) and Toll-like receptor (TLR)2 knockout (KO) mice (TLR2
),1 week after unilateral axotomy. (A,C) Contralateral side of the lumbar spinal cord; note the increased basal reactivity in WT compared with TLR2−/−. (B,D) The lesion upregulated GFAP labeling in both groups, but there was higher expression in WT mice. (E) Graph representing the ipsilateral:contralateral ratio of the integrated density of pixels. (F) Graph representing the quantification of the integrated density of pixels on unlesioned and lesioned sides ** P<0.01 and *** P< 0.001. (G,H) Purified astrocyte cultures showing astroglial reactivity in WT and TLR2−/− cultures. (I) Graph showing no significant difference in glial fibrillary acidic protein (GFAP) immunolabeling between the experimental groups. Scale bar: 50 μm.