Diapocynin protects nigrostriatum in MPTP-treated mice. (A) Seven days after MPTP treatment mice were sacrificed, tyrosine hydroxylase (TH)-3,3'-diaminobenzidine (DAB) immunostaining in the striatum and substantia nigra (SN) regions was performed (2× magnifications). (B) Representative Western blots illustrating the expression of TH in SN and striatum. Bar graphs showing mean Western blot TH/β-actin ratios ± SEM in (C) striatum and (D) SN of 6 mice per group. (E) Stereological counts of TH-positive dopaminergic neurons in the SN of ventral midbrain. Data are means ± SEM of 8 to 10 mice per group. ***
P <0.001 vs the control group; **
P <0.01 vs the MPTP group; #
P <0.001 vs the MPTP group.