Decreased numbers of neutrophils in the spinal cord and brain of COAM-treated mice. (A) Flow cytometry analysis for the indicated markers was used to identify leukocytes isolated from the spinal cord and brain. In this experiment cells were isolated on day 16 after immunization and stained for CD11b and Gr-1. Numbers indicated on each graph represent the percentages of CD11b+Gr-1+ and CD11b+Gr-1- cells. Each plot represents a staining pattern of pooled cells from three mice of one out of four representative experiments. (B) Morphologic examination of spinal cord and brain cytospin preparations stained with Hemacolor on day 16 after immunization after treatment with COAM. Bars represent averages ± SEM of at least three analyses; numbers of analyses are indicated between brackets; in each experiment cells from two mice were pooled per group. COAM, chlorite-oxidized oxyamylose; SEM, standard error of the mean.