Figure 5

Inflammatory chemokine expression in the CNS of hyperacute EAE control and COAM-treated mice. Mice were injected i.p. with a dose of 2 mg of COAM on day 0 and 7 after induction of EAE. CNS cells were harvested on day 11 or 12 post immunization. Fold mRNA levels of the CXC chemokines (A) KC, (B) MIP-2, (C) GCP-2 and (D) IP-10, and of the CC chemokines (E) MCP-1, (F) MIP-1α and (G) RANTES were determined by qPCR. Histograms and dots indicate, respectively, group medians and range of individual data points (for each cohort the number of mice (n) exceeded 10, 13≤n≤18). Differences between COAM-treated EAE mice, untreated EAE controls and naive mice were statistically analyzed by the Mann Whitney test. Alterations in control hyperacute EAE mice or COAM-treated hyperacute EAE mice versus naive mice are indicated on top of every cohort. Pairwise significant comparisons between saline-treated control and COAM-treated hyperacute EAE mice are indicated by horizontal lines above the compared groups. * P <0.05; ** P <0.01; *** P <0.001. CNS, central nervous system; COAM, chlorite-oxidized oxyamylose; EAE, experimental autoimmune encephalomyelitis.