GCP-2 and KC are upregulated in the peritoneum in response to COAM. SJL/J mice were induced for EAE by injection with SCH and treated with COAM (2 mg i.p., day 0 and 7 post immunization) or a control solution of saline. At different time points after COAM injection, peritoneal fluid was collected by i.p. lavage. Protein levels of GCP-2 and KC in peritoneal fluids were detected by ELISA. The total number (n) of mice examined per group are indicated between brackets; histograms indicate group means ± SEM. Compared to the naive levels, significant P-values of (*) P <0.05 were obtained (Mann–Whitney test). Pairwise comparisons between saline- and COAM-treated hyperacute EAE mice are indicated above the histograms. GCP-2 and KC levels in all naive mice (without EAE induction) were below the detection limit. COAM, chlorite-oxidized oxyamylose; EAE, experimental autoimmune encephalomyelitis; SCH, spinal cord homogenate; SEM, standard error of the mean.