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Table 2 COAM does not induce IFN-β

From: Rescue from acute neuroinflammation by pharmacological chemokine-mediated deviation of leukocytes

Incubation time COAM (μg/ml)
  1 10 30 100 300 1,000 control
4 hours ND ND ND 1.17 ± 0.20 ND 0.91 ± 0.31 1.00 ± 0.12
8 hours ND ND ND 1.33 ± 0.35 ND 1.07 ± 0.11 1.00 ±
0.1
16 hours ND ND ND 0.98 ± 0.18 ND 1.62 ± 0.46 1.00 ± 0.25
24 hours 1.12 ± 0.13 0.90 ± 0.15 0.19 ± 0.06 0.69 ± 0.37 0.17 ± 0.03 1.32 ± 0.65 1.00 ± 0.29
48 hours 1.11 ± 0.19 0.52 ± 0.09 1.92 ± 0.05 1.44 ± 0.25 1.55 ± 0.66 0.65 ± 0.39 1.00 ± 0.22
72 hours 1.28 ± 0.26 0.47 ± 0.14 1.43 ± 0.06 1.38 ± 0.38 1.5 ± 0.62 1.86 ± 0.59 1.00 ± 0.41
  1. Primary SJL/J mouse embryonic cells were incubated with different concentrations of COAM (1 to 1,000 μg/ml) for various time intervals. After the indicated incubation period, cells were lysed and total cellular RNA was subjected to qPCR. IFN-β mRNA expression levels were measured and normalized against untreated controls. Data represent mean values of fold IFN-β mRNA level ± SEM of two independent experiments (n=2-4). COAM, chlorite-oxidized oxyamylose; ND = not determined; SEM, standard error of the mean.