Skip to main content

Table 2 COAM does not induce IFN-β

From: Rescue from acute neuroinflammation by pharmacological chemokine-mediated deviation of leukocytes

Incubation time

COAM (μg/ml)

 

1

10

30

100

300

1,000

control

4 hours

ND

ND

ND

1.17 ± 0.20

ND

0.91 ± 0.31

1.00 ± 0.12

8 hours

ND

ND

ND

1.33 ± 0.35

ND

1.07 ± 0.11

1.00 ±

0.1

16 hours

ND

ND

ND

0.98 ± 0.18

ND

1.62 ± 0.46

1.00 ± 0.25

24 hours

1.12 ± 0.13

0.90 ± 0.15

0.19 ± 0.06

0.69 ± 0.37

0.17 ± 0.03

1.32 ± 0.65

1.00 ± 0.29

48 hours

1.11 ± 0.19

0.52 ± 0.09

1.92 ± 0.05

1.44 ± 0.25

1.55 ± 0.66

0.65 ± 0.39

1.00 ± 0.22

72 hours

1.28 ± 0.26

0.47 ± 0.14

1.43 ± 0.06

1.38 ± 0.38

1.5 ± 0.62

1.86 ± 0.59

1.00 ± 0.41

  1. Primary SJL/J mouse embryonic cells were incubated with different concentrations of COAM (1 to 1,000 μg/ml) for various time intervals. After the indicated incubation period, cells were lysed and total cellular RNA was subjected to qPCR. IFN-β mRNA expression levels were measured and normalized against untreated controls. Data represent mean values of fold IFN-β mRNA level ± SEM of two independent experiments (n=2-4). COAM, chlorite-oxidized oxyamylose; ND = not determined; SEM, standard error of the mean.
Back to article page

Journal of Neuroinflammation

ISSN: 1742-2094