Figure 2From: Dendritic cell CNS recruitment correlates with disease severity in EAE via CCL2 chemotaxis at the blood–brain barrier through paracellular transmigration and ERK activation Dendritic cells are largely perivascular indicating transmigration to CCL2 producing experimental autoimmune encephalomyelitis lesion areas. (A) Brain from Mouse 3 (Figure 1) stained with anti-CD31 and/or anti-chemokine ligand 2 (anti-CCL2) antibody and Hoechst 33342 dye. In all fields, CCL2 staining (green) was closely associated with myelin basic protein (MBP) staining (red). (B) Brain from Mouse 1 probed in vitro with anti-MBP antibody (lesions), anti-CD31 antibody (blood vessel endothelium) and Hoechst 33342 nuclear dye. The anti-CD11c signal represents ex vivo staining. In all fields, CD11c+ staining (white) was confined to perivascular tissue and did not extend beyond four or five cell layers, which is typical of this model [25]. (C) Dendritic cells are found in proximity to CCL2 in the central nervous system of mice with experimental autoimmune encephalomyelitis (EAE). Brain from Mouse 3 (Figure 1) stained with additional anti-CCL2 antibody (green), anti-CD68 (white) and Hoechst 33342 dye (blue). The anti-CD11c signal (red) represents ex vivo staining. CD11c+ dendritic cells (DCs) were always in clusters and were associated with depositions of CCL2. Data represent images from multiple tissue sections. Bar = 50 μm.Back to article page