Dendritic cells are largely perivascular indicating transmigration to CCL2 producing experimental autoimmune encephalomyelitis lesion areas. (A) Brain from Mouse 3 (Figure
1) stained with anti-CD31 and/or anti-chemokine ligand 2 (anti-CCL2) antibody and Hoechst 33342 dye. In all fields, CCL2 staining (green) was closely associated with myelin basic protein (MBP) staining (red). (B) Brain from Mouse 1 probed in vitro with anti-MBP antibody (lesions), anti-CD31 antibody (blood vessel endothelium) and Hoechst 33342 nuclear dye. The anti-CD11c signal represents ex vivo staining. In all fields, CD11c+ staining (white) was confined to perivascular tissue and did not extend beyond four or five cell layers, which is typical of this model
. (C) Dendritic cells are found in proximity to CCL2 in the central nervous system of mice with experimental autoimmune encephalomyelitis (EAE). Brain from Mouse 3 (Figure
1) stained with additional anti-CCL2 antibody (green), anti-CD68 (white) and Hoechst 33342 dye (blue). The anti-CD11c signal (red) represents ex vivo staining. CD11c+ dendritic cells (DCs) were always in clusters and were associated with depositions of CCL2. Data represent images from multiple tissue sections. Bar = 50 μm.