Figure 3

Rod microglia couple together to form trains. Immunofluorescence labeling of Iba1-positive microglia (red) with the nuclear marker Hoechst (blue) revealed that aligned rod microglia at 7 days post-injury were composed of multiple cells aligned in a single trajectory with their highly polarized processes seemingly intertwined. These rod microglia coupled together to form long trains which spanned multiple cortical layers.