Stations do not involve glial cells. (A) In sham-injured rats, only ramified microglia (red) were observed with no association to normal CNPase stained oligodendrocytes (green). In TBI, there was evidence of decreased CNPase staining intensity at day 7 post TBI when rod microglial cells were present (Hoechst nuclei staining blue). (B) GFAP-positive astrocytes (green) were present amongst ramified microglia (red) in sham-injured animals. Following injury, astrocytes became hypertrophic and appeared in conjunction with rod microglia. Astrocytes showed no alignment with trains of rod microglia. (C) Confocal analysis determined that there was no co-localization of GFAP-positive astrocytes with rod microglial cells.