Induction of neuroborreliosis following a single inoculation with
. Serum levels of IgM towards B. burgdorferi were measured at 28 days post-inoculation in naïve and infected rats, panel (A). recA copy number was measured to determine the magnitude of brain infection in naïve and infected rats, panel (B) and immunohistochemistry was performed to identify the presence of B. burgdorferi in an infected rat brain at 28 days post-inoculation, panel (C). Values represent the means ± SD of serum immunoreactivity, or brain copy number with a sample size of six representing samples from six different rats per group. The asterisk (*) represents a significant difference (P ≤0.05) comparing infected to naïve rats using an unpaired t test (GraphPad InStat, version 3.10).