Figure 7

Representative histological stainings from cerebella of chimeric mice with EAE. Left panels, [Prnp ^-/- >Prnp ^+/+] mouse. Right panels, [Prnp ^+/+ >Prnp ^-/-]. (A-B) hematoxylin/eosin staining showing white matter perivascular cellular infiltrates (black arrows) that were more spread in [Prnp ^+/+ >Prnp ^-/-] than in [Prnp ^-/- >Prnp ^+/+] mice. (C-D) Bielschowsky silver impregnation staining in the white matter, showing reduced axonal staining in [Prnp ^+/+ >Prnp ^-/-] mouse as compared to [Prnp ^-/- >Prnp ^+/+] mouse, despite the presence of an inflammed vessel in the center in both examples. (E-F) Luxol Fast blue staining showing a clear-cut demyelinating area (white arrow) in [Prnp ^+/+ >Prnp ^-/-] mouse as compared to the normal appearing white matter in [Prnp ^-/- >Prnp ^+/+] mouse. The dashed lines point to the white matter/grey matter limit. wm, white matter. Scale bars: 100 μm.