Microglial podosomes are enriched in the CRAC/Orai1 channel and closely associated with STIM1. All panels show high-resolution, deconvolved images. (A) The podonut region of a representative microglial cell is stained for Orai1 (green) and the podosome core marker, Arp2 (red); merged image is at the right. Scale bar, 5 μm. (A’) High-magnification images from the boxed region show strong co-localization of Orai1 and Arp2 in podosome cores. Scale bar, 1 μm. (B) Color-separated and merged images of a microglial cell stained for STIM1 (green), vinculin (red), and the nuclear stain, DAPI (blue). Note the vinculin enrichment in the podonut. Scale bar, 5 μm. (B’) High-magnification images from a different cell show proximity of STIM1 and vinculin, and several examples of co-localization (arrows). Scale bar, 1 μm.