Table 1 Antibodies
Primary antibodies (species and type) | Dilution | Supplier |
|---|---|---|
α-Tubulin (mouse monoclonal) | 1:1000 | Abcam (Cambridge, MA, USA) |
Arp2 (rabbit polyclonal) | 1:100 | Santa Cruz Biotechnology (Santa Cruz, CA, USA) |
CaM (rabbit monoclonal or mouse monoclonal) | 1:200 | Abcam |
Iba-1 (rabbit polyclonal) | 1:1000 | Wako Chemicals (Richmond, VA, USA) |
Orai-1 (goat polyclonal) | 1:100 | Santa Cruz Biotechnology |
SK3 (rabbit polyclonal) | 1:200 | Alomone (Jerusalem, Israel) |
STIM1 (rabbit polyclonal) | 1:100 | Sigma-Aldrich (St. Louis, MO, USA) |
Talin1/2 (mouse monoclonal) | 1:100 | Abcam |
TRPM7 (goat polyclonal) | 1:200 | Santa Cruz Biotechnology |
Vimentin (mouse monoclonal) | 1:1000 | Millipore (Billerica, MA, USA) |
Vinculin (mouse monoclonal) | 1:200 | Sigma-Aldrich |
Secondary antibodies | ||
DyLight 488 or 594 (donkey anti-rabbit) | 1:500 | Jackson Immunoresearch (West Grove, PA, USA) |
DyLight 488 or 594 (donkey anti-mouse) | 1:500 | Jackson Immunoresearch |
DyLight 488 (donkey anti-goat) | 1:500 | Jackson Immunoresearch |