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Figure 2 | Journal of Neuroinflammation

Figure 2

From: Upregulation of mesencephalic astrocyte-derived neurotrophic factor in glial cells is associated with ischemia-induced glial activation

Figure 2

Expression of mesencephalic astrocyte-derived neurotrophic factor in oligodendrocytes. Mesencephalic astrocyte-derived neurotrophic factor (MANF) was expressed in normal cerebral cortex (A to C) and ischemic cerebral cortex (D to J). The primary cultured oligodendrocytes were treated with vehicle (DMEM medium containing 5% serum) (K to N) or tunicamycin (1 μg/ml) (O to R). Twenty-four hours after treatment, immunofluorescent staining was performed. The oligodendrocytes were identified with anti-2-3-cyclic nucleotide 3-phosphodiesterase (anti-CNP) (red, B, E, I, M, and Q). MANF was detected with anti-MANF antibody (green, A, D, H, L, and P). Scale bar = 50 μm. (S) Percentage of MANF-positive cells in neural cells. The number of MANF-positive cells and the number of double positive cells co-expressing glial fibrillary acidic protein (GFAP), CD68, CNP, or NeuN were counted in five randomly selected fields under a high-power field (×400 magnification). The percentage of MANF expressing cells in the population of astrocytes, microglial, and oligodendrocytes was calculated.

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