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Figure 3 | Journal of Neuroinflammation

Figure 3

From: Upregulation of mesencephalic astrocyte-derived neurotrophic factor in glial cells is associated with ischemia-induced glial activation

Figure 3

Induction of mesencephalic astrocyte-derived neurotrophic factor expression in the primarily cultured astrocytes. Cells cultured in the DMEM medium containing 5% serum were used as controls (A to C). Glial cells were cultured as described in Materials and methods and treated with 1 μg/ml tunicamycin (D to F), 10 μM MG132 (G to I), and serum-free DMEM medium (J to L), respectively. Twenty-four hours after treatment, the astrocytes were identified with anti-glial fibrillary acidic protein (anti-GFAP) antibody (red, A, D, G, and J). Mesencephalic astrocyte-derived neurotrophic factor (MANF) expression was detected with monoclonal anti-MANF antibody (green, B, E, H, and K). Nuclei were stained with 4′,6-diamidino-2-phenylindole (blue). The arrows show MANF immune-positive astrocytes after treatment. Scale bar = 50 μm.

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