Figure 3

Expression of complement-related genes in the retina by quantitative polymerase chain reaction (qPCR) following 670-nm light treatment and bright continuous white light (BCL) exposure. (A,B) The expression of complement components C1s, C2, C3, C4b (A) and receptors C3aR1, C5r1 (B) increased significantly following BCL relative to dim-reared animals (P <0.05). In animals treated with 670-nm light prior to BCL exposure, the expression of all complement-related genes assessed was substantially reduced, compared to those exposed to BCL alone (P <0.05). Dim-reared animals exposed 670-nm light showed no significant modulation of complement gene expression relative to controls (P >0.05). Dim reared n = 3, dim reared + 670 nm n = 3, light damage n = 3, light damage + 670-nm light n = 3; error bars represent SEM. *Significant change using analysis of variance (ANOVA) with Tukey’s post test where P <0.05.