Figure 5

Immunoreactivity (IR) for C3 (red) in the retina following 670-nm light treatment and exposure to bright continuous white light (BCL). (A-F) Representative images of C3 immunoreactivity taken from the superior mid-periphery. (A,B) IR for C3 was faintly detected in retinal vasculature (arrows) of dim-reared retinas (A), as well as those treated with 670-nm light (B), at comparable levels of IR. (C-F) Following BCL exposure, IR for C3 was observed in multiple deposits throughout the outer nuclear layer (ONL) in the lesion area (C-D, arrows) and the layer of outer segments at the edge of the lesion (E-F, arrows). (G) C3-IR was vastly reduced in the ONL and outer segments of animals treated with 670-nm light prior to BCL, and appeared similar to dim-reared controls (arrows). (H) Negative controls showed no specific staining for C3 following BCL exposure. (I-L) C3-IR (red) in the outer segments of sections counterimmunolabelled with anti-4-hydroxynonenal (4-HNE) (green), shows colocalization for aggregations of 4-HNE immunoreactivity (arrows) following BCL. C = choroid; ONL = outer nuclear layer; OS = outer segment layer.