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Figure 7 | Journal of Neuroinflammation

Figure 7

From: Metabotropic glutamate receptor 5 contributes to inflammatory tongue pain via extracellular signal-regulated kinase signaling in the trigeminal spinal subnucleus caudalis and upper cervical spinal cord

Figure 7

Neuronal localization and expression of mGluR and effects of continuous i.t. administration of the mGluR5 antagonist, MPEP, on CFA-induced ERK phosphorylation. Photomicrographs of mGluR5 (A) and NeuN (B) staining in the Vc following noxious mechanical stimulation of the tongue on day 8 after CFA injection. (C) Overlay of the double imunofluoresence labeling, showing the neuronal nature of mGluR5-IR cells. Arrows indicate the double-labeled cells. (D, E, F) Photomicrographs of mGluR5 (D) and pERK (E) staining in the Vc on day 8 after CFA injection. (F) Overlay of the double imunofluoresence labeling, suggesting the colocalization of mGluR5 with pERK (arrows). (G) Normalized amount of mGluR5 protein in Vc and C1-C2 on day 8 after saline or CFA injection into the tongue. β-actin was used as a loading control. The mean number of noxious stimuli-triggered pERK-IR cells in the ipsilateral (H) and contralateral (I) Vc and C1-C2 on day 8 after CFA injection into the tongue with continuous i.t. administration of MPEP or saline. MPEP administration effectively decreased CFA-induced pERK upregulation. n = 4 in each group. Error bars indicate SEM.

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Journal of Neuroinflammation

ISSN: 1742-2094