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Table 1 Effects of FcγR−/− on α-SYN-induced pro-inflammatory molecules

From: The gamma chain subunit of Fc receptors is required for alpha-synuclein-induced pro-inflammatory signaling in microglia

Fold 4 h 24 h
WT vehicle WT α-SYN FcγR−/−vehicle FcγR−/−α-SYN WT vehicle WT α-SYN FcγR−/−vehicle FcγR−/−α-SYN
IL-1α 1.00±0.48 3.32±2.45 1.00±0.21 0.55±0.22 1.00±0.17 1.05±0.23 1.00±0.20 1.03±0.42
IP-10 1.00±0.21 5.29±2.93 1.00±0.41 0.79±0.38 1.00±0.23 1.01±0.34 1.00±0.07 1.79±0.42
MIP-1α 1.00±0.21 3.35±0.61a 1.00±0.11 0.97±0.39 1.00±0.09 1.29±0.35 1.00±0.31 2.07±0.93
MIP-1β 1.00±0.23 4.54±0.56a 1.00±0.15 1.19±0.45 1.00±0.08 1.26±0.38 1.00±0.46 2.91±1.55
MIP-2   nd   1.00±0.20 1.37±0.21 1.00±0.46 1.40±0.75
MCP-1   nd   1.00±0.12 1.24±0.37 1.00±0.31 1.88±1.10
  1. Conditioned media were collected 4 h and 24 h after the treatment of vehicle or aggregated human α-SYN on WT and FcγR−/− microglia for a 25-plex mouse cytokine/chemokine assay. At 4 h, MIP-1α and MIP-1β were significantly increased with α-SYN treatment in WT microglia but not FcγR−/− microglia compared with vehicle-treated ones. All cytokine and chemokine expression levels were normalized to the level of vehicle-treated WT or FcγR−/− microglia, respectively.
  2. a P <0.001 WT α-SYN vs. WT vehicle. ANOVA with Tukey’s multiple comparison test. nd, not detectable.