Figure 5

Corticosterone- and aldosterone-mediated NF-κB translocation is dependent on MR . BV-2 cells were treated for 24 h with vehicle (A), 50 ng/mL TNF-α (B), 25 nM corticosterone (C), 25 nM corticosterone and 1 μM spironolactone (D), 25 nM corticosterone and 250 nM Cay-10512 (E), 50 nM aldosterone (F), 50 nM aldosterone and 1 μM spironolactone (G), or 50 nM aldosterone and 250 nM Cay-10512 (H). The localization of the p65 subunit of NF-κB was visualized by immunofluorescence microscopy using a primary antibody against p65 and a secondary ALEXA fluor 488 labeled antibody (magnification 400×).