Platelet-derived growth factor (PDGF)-BB-mediated induction of monocyte chemoattractant protein 1 (MCP-1) expression involves nuclear factor κB (NFκB) activation. (A) Exposure of A172 cells to PDGF-BB resulted in a time-dependent increase in phosphorylation of the p65 subunit of NFκB in the nuclear cellular fraction. Reciprocally, PDGF-BB exposure resulted in a time-dependent increase in inhibitor of κBα (IκBα) phosphorylation in the cytosolic cellular fraction of A172 astrocytes. (B) Pretreatment with the IκBα inhibitor, SC514 resulted in inhibition of PDGF-BB-mediated induction of MCP-1. (C) Transduction with mutant-NFκB but not wild-type (WT)-NFκB resulted in abrogation of PDGF-BB-mediated induction of MCP-1. All the data are presented as mean ± SD of three individual experiments. ***P <0.001 versus control group, #P <0.05, ###P <0.001 versus PDGF-BB-treated group. (D) Schematic illustration of NFκB binding consensus sequence on the promoter region of MCP-1. (E) Chromatin immunoprecipitation (ChIP) assay demonstrating PDGF-BB-mediated binding of p65NFκB to the MCP-1 promoter.