Age- and hormone-related changes in mRNA expression of genes related to phagocytosis. Real-time PCR demonstrated age- and ovariectomy-related increase in the expression of Cd32 (A), Msr2 (C), C1qb (E), C3 (F), and decrease of Cd93 (B), Cd36 (D). Error bars show SD of six biological samples. Statistical significance of the alterations in different groups compared to middle-aged female rats (M) was analyzed using analysis of variance (ANOVA) followed by Newman-Keuls post hoc test. Asterisks indicate changes with statistical significance: *corresponds to 0.01<P < 0.05, ** to 0.001<P < 0.01 and *** to P < 0.001. Y, young rat; M/OVX, middle-aged ovariectomized (OVX) rat; M/OVX+E2, middle-aged OVX rat with 17β-estradiol (E2) replacement.